Peripheral Blood Biospecimens are a critical tool in immunology research due to their significant role in protecting and maintaining healthy bodily functions. We use the term “Peripheral Blood” to describe a broad category of human biospecimens that are related to the human body’s circulatory system.
Peripheral blood is the primary method for transporting nutrients such as oxygen and carbon dioxide through the body and has 3 primary components: erythrocytes (red blood cells), leukocytes (white blood cells), and thrombocytes (blood platelets). The medium which allows these components to flow through the body is called blood plasma. Erythrocytes, or red blood cells, are the primary transportation mechanism for oxygen throughout the human body. In normal (healthy) samples, the mature form of the cell is non-nucleated, reddish, biconcave disk. Each of these cells contains hemoglobin, the iron-containing metalloprotein that allows for oxygen-transport. Leukocytes, or white blood cells, have a crucial role in the immune system and are meant to protect the body against diseases. In normal samples, they are a colorless blood corpuscle capable of ameboid movement. Blood platelets (thrombocytes) are the irregularly shaped disk-like particles in the blood and have no defined nucleus, DNA, or hemoglobin. Blood platelets contain active enzymes and mitochondria and are therefore primarily responsible for blood coagulation. Blood Plasma is the clear, yellowish fluid portion of blood, lymph, or intramuscular fluid in which these cellular components are suspended.
At Conversant Bio, we offer a wide variety of human peripheral blood samples from normal donors and from patients with cancer or autoimmune/inflammatory disorders. Some of the most popular peripheral blood products include our peripheral blood mononuclear cells, peripheral blood isolated B-Cells, and peripheral blood viable plated cells. To learn more about our individual peripheral blood products browse the items below.
Peripheral Blood Products
Peripheral Blood | Mononuclear Cells
Mononuclear cells, mainly consisting of monocytes and lymphocytes, are obtained by gradient centrifugation using Ficoll. These cells are aliquoted and cryopreserved at -150C in a manner that maintains post-thaw cellular viability.
Key Applications:
- drug testing and drug target discovery
- assessing immune responses
- toxicology studies
- genetic studies
- xenografting
- cancer stem cells
- cell isolation studies
Peripheral Blood | CD45
These hematopoietic cells are obtained through positive selection involving antibody binding of cell-surface CD45 and magnetic column-based isolation. This results in CD45+ hematopoietic cells that are >90% pure by flow cytometry.
Key Applications:
- drug testing and drug target discovery
- assessing immune response
- toxicology
- genetic studies
- xenografting
- cancer stem cells
Peripheral Blood | T-Cells, CD3
These T-cells are obtained through positive selection involving antibody binding of cell-surface CD3 and magnetic column-based isolation. This results in CD3+ T-cells that are >90% pure by flow cytometry.
Key Applications:
- drug testing and drug target discovery
- assessing immune response
- toxicology
- genetic studies
Peripheral Blood | T-Cells, Negative Selection
These T-cells are obtained through negative selection involving binding of non-T-cells and magnetic column-based isolation. This results in T-cells without antibodies bound to their cell surface ("untouched") that are >90% pure by flow cytometry.
Key Applications:
- drug testing and drug target discovery
- assessing immune response
- toxicology
- genetic studies
Peripheral Blood | B-Cells, CD 19
These B-cells are obtained through positive selection involving antibody binding of cell-surface CD19 and magnetic column-based isolation. This results in CD19+ B-cells that are >90% pure by flow cytometry.
Key Applications:
- drug testing and drug target discovery
- assessing immune response
- toxicology
- genetic studies
Peripheral Blood | B-Cells, Negative Selection
These B-cells are obtained through negative selection involving binding of non-B-cells and magnetic column-based isolation. This results in B-cells without antibodies bound to their cell surface ("untouched") that are >90% pure by flow cytometry.
Key Applications:
- drug testing and drug target discovery
- assessing immune response
- toxicology
- genetic studies
Peripheral Blood | Viable Plated Cells
Cells, fresh or previously cryopreserved, can be plated on any desired plates or flasks and then shipped the same day for overnight delivery. Plates containing cells are heat-sealed prior to shipping to minimize leakage between wells. This option is ideal for researchers wanting to place cells in their desired assay conditions as quickly as possible or when concerned with properly thawing cryopreserved primary samples.
Key Applications:
- drug testing and drug target discovery
- assessing immune response
- toxicology
- genetic studies
Peripheral Blood | Circulating Tumor Cells
Fresh peripheral blood (shipped the same day of draw for overnight delibvery) from patients with known circulating tumor cells as determined by Veradex.
Key Applications:
- biomarker identification/ validation
- genetic studies
- cell isolation
- drug target discovery
Peripheral Blood | Buffy Coat
Mononuclear cells, mainly consisting of monocytes and lymphocytes, are obtained by gradient centrifugation using Ficoll. These cells are aliquoted and frozen at -80C in a manner that does not maintain post-thaw cellular viability.
Key Applications:
- biomarker identification/ validation
- genetic studies
- drug target discovery
Peripheral Blood | Plasma
Plasma is the liquid, non-cellular portion of blood. It contains a variety of proteins, nutrients, ions, hormones and clotting factors.
Key Applications:
- biomarker identification/ validation
- genetic studies
Peripheral Blood | Serum
Serum is the liquid, non-cellular portion of blood lacking clotting factors. It contains a variety of proteins, nutrients, ions, and hormones.
Key Applications:
- biomarker identification/ validation
- genetic studies
Peripheral Blood | Special Processing
Peripheral blood can be processed in a manner that best suits your research. Please provide us with a detailed description of how you will like the sample handled. To inquire about our special processing services please click the icon to the right and you will be directed to our contact page. There you will be able to fill out a form and describe the specific samples you are seeking.
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