Support Cells
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Mesenchymal stem cells, or MSCs, are multipotent stem cells that can differentiate into a variety of cell types, including: myofibroblasts (between a fibroblast and smooth muscle cell), osteoblasts (bone cells), chondrocytes (cartilage cells) and adipocytes (fat cells). MSCs are characterized morphologically by a small cell body with a few cell processes that are long and thin. A Myofibroblast is a cell that is in between a fibroblast and a smooth muscle cell in differentiation. Myofibroblasts and MSCs have similar morphological characteristics. There are many possible ways of myofibroblast development. These include direct myofibroblastic differentiation of a progenitor cell resident in a stromal tissue or homing and recruitment of a circulating MSC that then undergoes differentiation. Myofibroblasts have been shown to resemble cancer associated fibroblasts in their ability to support tumor progression and growth. |
| Cancer associated fibroblasts, or CAFs, are fibroblast found within tumors or in contact with tumor cells that play a critical role in the progression, growth, invasion, and metastasis of cancer. At Conversant Bio, we offer several types of support cells derived from normal and diseased donors. These cells are isolated from biospecimens obtained from our extensive clinical network and minimally cultured in vitro. These flow cytometry characterized cells are ideal for in vitro and in vivo mixed cell population studies. If you are interested in obtaining a specific biospecimen or don’t see what you are looking for please click the "have questions?" icon below and someone from our team will contact you shortly. Because Patients Are Waiting… |
support cell product types
Mesenchymal Stem Cells | Bone Marrow Mononuclear CellsMesenchymal stem cells (MSC) are isolated from the bone marrow of non-diseased donors or multiple myeloma patients. These cells are minimally cultured for 2-5 passages and cryopreserved in an animal protein free media containing 10% DMSO at ~1 million cells per vial. The percentage of viable cells is >70% post thaw. Alternatively, cells can be provided fresh in a >70% confluent T-25 flask. MSCs are characterized with the following cell-surface markers: >90% CD105+, CD90+, and/or CD44+ and <10% CD326+, CD45+, CD31+, CD133+, and/or CD34+. Although not routinely tested, <10% of cells in our tested MSC samples express low levels of intra-cellular alpha-smooth muscle actin.key applications:
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MyofibroblastsMyofibroblasts are differentiated from MSC isolated from non-diseased donors. These cells are minimally cultured for 2-5 passages and cryopreserved in an animal protein free media containing 10% DMSO at ~1 million cells per vial. The percentage of viable cells is >70% post thaw. Alternatively, cells can be provided fresh in a >70% confluent T-25 flask. Myofibroblasts are characterized with the following cell-surface markers: >90% CD105+, CD90+, and/or CD44+ and <10% CD326+, CD45+, CD31+, CD133+, and/or CD34+. Over 80% of cells in our myofibroblast samples express intra-cellular alpha-smooth muscle actin.key applications:
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Cancer Associated FibroblastsCancer associated fibroblasts (CAF) are isolated from tumor samples obtained from our large network of clinical sites. These cells are minimally cultured for 2-5 passages and cryopreserved in an animal protein free media containing 10% DMSO at ~1 million cells per vial. The percentage of viable cells is >70% post thaw. Alternatively, cells can be provided fresh in a >70% confluent T-25 flask. CAFs are characterized with the following cell-surface markers: >90% CD105+, CD90+, and/or CD44+ and <10% CD326+, CD45+, CD31+, CD133+, and/or CD34+. Although not routinely tested, >80% of cells in our tested CAF samples express intra-cellular alpha-smooth muscle actin.key applications:
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