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Human Tissue Sample Blog

Your source for information about acquiring human biospecimens for your research.

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4 Methods for Extracting DNA from FFPE Tissue Samples

Extracting DNA from FFPE Tissue Samples

Tissues stored for long time periods are typically fixed with formalin to preserve the tissue’s protein structure and then embedded in paraffin wax so that the tissue can be readily prepared in thin slices. This technique, known as formalin-fixed paraffin-embedded (FFPE), has been invaluable for providing well-annotated biospecimens for large-scale drug discovery and biotech research.  This is because, for many research protocols, there is often a lack of adequate numbers of fresh tissue samples for projects such as cancer biomarker research.

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How to Extract Nucleic Acids from FFPE Tissue Samples

How to Extract Nucleic Acids from FFPE Tissue Samples

About a century ago, an excellent method of preserving tissue samples was developed. That technique, known as Formalin-Fixed, Paraffin-Embedded (FFPE) tissue, was a step forward for biospecimen research. However, FFPE was developed primarily for histological applications. Today, there is great interest in using FFPE biospecimens for molecular biological research.

Can You Isolate RNA from Your FFPE Tissue Samples?

Can-You-Isolate-RNA-From-Your-FFPE-Tissue-Samples

Today, millions of formalin-fixed paraffin-embedded (FFPE) tissue specimens are archived worldwide, along with valuable annotation data that aids current researchers seeking better understanding and treatments for serious diseases that still resist a permanent cure. In fact, high-quality FFPE tissues are critical to successful biomedical research.  However, while FFPE biospecimens are good sources for certain analyses, they do have limitations. The process of fixing the biospecimen, then embedding it in paraffin causes a cross-linkage between nucleic acids and proteins, which makes it difficult to use for research requiring tissue RNA. Happily, there are a variety of methods today that can extract viable RNA for a number of important in vitro studies.

What are the Different Stages of Rheumatoid Arthritis?

What-Are-The-Stages-of-Rheumatoid-Arthritis

Rheumatoid arthritis (RA) is a systemic, chronic inflammatory autoimmune disease that primarily impacts the synovium of joints, especially those characterized by freely movable articulations (i.e. diarthrodial). RA affects three times as many women as men, and typically exhibits in the fourth to sixth decade of life. The underlying genetic basis for RA is not yet well understood, but most researchers believe that RA is caused by a combination of genetic and environmental triggers, with genetic factors being responsible for a 50 percent risk of developing the disease.

Can Biomarkers Detect Mild Rheumatoid Arthritis Sooner?

rhuematoid arthritis feet

Rheumatoid arthritis (RA) is an autoimmune disease with characteristic symptoms of joint swelling and pain. Approximately 60 to 70 percent of RA patients have the chronic form of RA, which over time, causes worsening symptoms, eventually leading to joint damage and sometimes systemic complications.

How is Seropositive Rheumatoid Arthritis Diagnosed?

How-is-Seropositive-Rheumatoid-Arthritis-Diagnosed?

Rheumatoid arthritis (RA) is an immune system disease whichattacks the tissues in the body. Early symptoms of RA affect joints in the hand, wrist, knees,ankles, and feet and include pain, swelling, and stiffness of the joints. Severe symptoms include loss of joint movement and even joint deformity. It is possible for the disease to go into remission causing the pain and swelling in early stages to disappear. However, those symptoms will appear again later.

Who is Commonly Affected by Systemic Lupus Eyrthematosus (SLE)?

who gets lupus

Systemic lupus erythematosis is a serious and chronic autoimmune disease that affects multiple organ systems, most predominantly the skin and joints. SLE varies in severity, depending on the organ systems involved. Diagnosis is difficult, and is made on the basis of results of a variety of blood tests, urinalysis, as well as the presence of distinguishing disease features, such as skin rash, photosensitivity, oral ulcers, and renal disorder.

7 Types of Blood Tests Used to Diagnose Lupus

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Lupus can be a difficult disease to definitively diagnose, which is why physicians typically order a wide battery of blood tests for patients who may be suffering from this inflammatory autoimmune disorder. In patients with lupus, their immune system, which should protect their body from infections, instead incorrectly produces antibodies against the person’s own tissues. A diagnosis of lupus is a serious matter, as it is a chronic disease that, over time, often causes damage to key organ systems, including the kidneys, heart, lungs, skin and brain. Several types of lupus exist, but the most common form is known as systemic lupus erythematosus (SLE).

4 Benefits of Bone Marrow for Lupus Treatment Research

bone marrow for lupus

Human bone marrow serves as the factory for developing mesenchymal stem cells, as well as the birthplace of the human immune system. Because so many cell progenitors are located in the marrow, it is an invaluable tool that can help researchers learn more about the sources of blood and autoimmune disorders.

Can Non-toxic Peptides Put Lupus into Remission?

nontoxic peptides lupus remission

Systemic lupus erythematosus, commonly known as lupus or SLE, is a serious and chronic autoimmune disease characterized by inflammation across the body’s organ systems. The organs most commonly impacted are the skin, joints, kidneys, blood cells and nervous systems. As with most autoimmune disorders, lupus causes the body to attack its own tissues, because of a breakdown in the body’s immune system that causes it to stop differentiating between healthy and diseased tissues. There are four main types of lupus, with 70 percent of people having the SLE lupus type. It’s estimated that 1.5 million Americans have lupus, and 16,000 new cases are diagnosed each year.

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