What Method Can Be Used to Create a Cancer Cell and PBMC Coculture?

Posted by Luke Doiron on Aug 27, 2015 10:46:50 AM

Recent findings from the cancer research community have identified the importance of cell-cell communications in the activation or inhibition of tumor development. Cellular co-cultures have become an important in-vitro methodology for gaining a better understanding of how cells interact in the cancer microenvironment. These insights are also fueling development of drug modulators that effectively target the cell-to-cell dialogue that may fuel cancer development or therapy resistance.

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How to Choose the Best Enzyme for Tumor Dissociation

Posted by Luke Doiron on Aug 25, 2015 9:11:00 AM

Does your research protocol require carefully-sourced tumor specimens for oncology studies? Knowing which isolation and dissociation procedures are appropriate to the particular tumor you're investigating is key so  that you distinguish all the cells found in the tumor from one another. Tumors are not composed of just one cell type, but are oftentimes highly heterogeneous; this means that you need to pick the right enzyme, or mixture of enzymes, to obtain good cellular yields, viability and well-separated cells.

Here are some general tips for utilizing enzymes for tissue dissociation research:

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3 Pitfalls of Isolating Monocytes from Human Peripheral Blood

Posted by Luke Doiron on Aug 20, 2015 11:03:56 AM

Peripheral blood finds wide use in biomedical research because of its role in supporting the human body's immune system and other key health functions. It is composed of three types of cells - erythrocytes (red blood cells), leukocytes (white blood cells) and thrombocytes (platelets).

Monocytes are one type of leukocyte, and develop into macrophages upon migration into human tissue from the bloodstream. Macrophages surround and digest pathogens and leave fragments on their surfaces as a signal, which is recognized by other immune cells. Monocytes and macrophages play important roles in immune defensive response, inflammation, and tissue remodeling.

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A Great Technique for Isolating DNA from FFPE Tissue

Posted by Luke Doiron on Aug 18, 2015 10:44:00 AM

Formalin-fixed paraffin-embedded (FFPE) tissue specimens have been an invaluable research resource for nearly a century, and today, a huge library of archival FFPE samples is available around the world. While historically, FFPE samples were valued pathology specimens, now they are finding wide application for molecular level analysis that drives important genetic studies, drug discovery, and immunotherapy research. Happily, techniques have been developed that overcome the challenge of DNA-tissue protein cross-links caused by formalin fixation and so allow for extraction of DNA from FFPE tissues.

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The 4 Best Cell Isolation Kits to Perform Tissue Dissociations

Posted by Luke Doiron on Aug 13, 2015 1:15:00 PM

Separation of viable, high-quality cells from tumor tissue or other types of biospecimens is a critical step in biomedical research and development. The results of your research are impacted in part by the quality of the starting biospecimen as well as the methodology used to dissociate the tissues and isolate cells found in that sample. Properly prepared cell mixtures that are high in viability, purity and yield give you a positive jump-start for quality yields of RNA, proteins and other cell structures; these in turn are invaluable for downstream uses such as isolating and identifying cancer biomarkers or gene expression analysis.

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How to Choose the Right Biobank for FFPE Tissue Samples

Posted by Luke Doiron on Aug 11, 2015 12:07:00 PM

Formalin-Fixed, Paraffin Embedded (FFPE) tissue specimens are found in biobanks around the world and provide an invaluable resource for scientists involved in biomedical research, large-scale drug discovery, genetic studies, and other important applications. One of the main reasons for their widespread availability and use is the fact that FFPE fixation is the most common technique for tissue archival preparation, having been an accepted method for preservation for nearly a century.

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5 Cell Isolation Techniques to Consider for Cancer Research

Posted by Luke Doiron on Aug 4, 2015 8:00:00 AM

Cells from patients suffering from cancer are invaluable research tools for drug discovery, cancer stem cell studies, immunotherapy protocols, and research into understanding the pathways of cancer metastasis. It’s become widely accepted that because tumors contain a large variety of heterogeneous cell populations, isolating and studying those cells is key to advancing cancer research. There are a number of methods that are traditionally used to isolate different cell types from a mixed suspension.  As well, there are new and innovative techniques being investigated to determine their utility in cancer research programs. Consider these five cell isolation techniques:

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What Parameters Impact the Outcome of Viable Dissociated Cells

Posted by Luke Doiron on Jul 29, 2015 3:11:00 PM

When cell isolation is the goal, selecting the right protocol can greatly influence cell viability and yield, and therefore impact your research data. This is why it's important to understand what parameters effect the outcome of cell dissociation processes, so you select the correct materials and methods for your desired endpoint and downstream application.

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