Alzheimer's Disease: How do you know, and how do you cope?

Posted by Luke Doiron on Jul 4, 2015 7:30:00 AM

We have been receiving feedback from readers stating that they would like to learn more about what patient's lives are like during the diagnosis and treatment phases of the diseases we work with.  Often, clues from treatment procedures will help indicate the availability of biospecimens. Over the next few weeks, look for a series of articles surrounding patient treatment and diagnosis as well as a few patient profiles.

Alzheimer's is a brain disease that causes a slow decline in memory, thinking and reasoning skills. There are ten warning signs from the Alzheimer’s Association that you may possibly be developing Alzheimer’s. Not all individuals will experience all of these signs, and some may experience them at varying degrees.

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How to Minimize Bleed-Through in Images of FFPE Sections

Posted by Luke Doiron on Jun 25, 2015 6:00:00 AM

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Is It Necessary to Thaw PBMCs in Their Expanding Media?

Posted by Luke Doiron on Jun 23, 2015 2:45:00 PM

Scientists conducting research in a wide variety of fields, including immunology, infectious diseases, stem cells, cancer, drug development, vaccine development, transplant immunology and cell-based assays often turn to PBMC specimens. Thousands of cryopreserved PBMC specimens are available in biobanks around the world, making them an important source for researchers. Fortunately, it's been found that cryopreserved  PBMCs are useful and valid specimens as long as they are properly handled.

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Understanding Crohn's Disease

Posted by Olivia Hendrick on Jun 18, 2015 4:30:00 PM

Many people have heard the name, but few understand what the disease really is. Crohn’s disease is a “chronic inflammatory bowel disease that affects the lining of the digestive tract.”  The disease was named after Burrill Crohn who was the first to diagnose this disease. Crohn’s disease is difficult to diagnose because its symptoms (which include fever, nausea, weight loss, diarrhea, fatigue, abdominal pains, and rectal bleeding) are similar to other autoimmune diseases such as lupus and type 1 diabetes. Crohn’s most commonly affects the end of the small intestine and the beginning of the colon, but it may affect any part of the gastrointestinal tract, from the mouth to the anus.

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How to Optimize Your Cell Isolation Method for Best Results

Posted by Luke Doiron on Jun 4, 2015 8:00:00 AM

The goal of cell isolation is to maximize the yield of high-viability dissociated cells for a variety of scientific applications, including tissue culture and cell biology research. There are many, many protocols for this process, because of the wide variation in desired endpoint, type of tissue, and required dissociation enzyme(s). So while we can't offer a simple optimization method, we can point out a few key factors that play a role in optimizing your specific cell isolation program.

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5 Best Practices for Working with Enzymes for Tissue Dissociation

Posted by Luke Doiron on Jun 3, 2015 10:03:39 AM

Biomedical research frequently relies on high-quality tissue specimens that are then enzymatically dissociated into single cell preparations. These cells are useful for a wide range of studies, such as gene expression profiles or analysis of cell surface markers, as well as for growing cell cultures using an isolated primary cell. Successful tissue dissociation with enzymes is affected by a variety of factors which can impact cell viability, yield, and ultimately, clinical usefulness.

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4 Techniques for Dissociation of Human Cancer Cells

Posted by Luke Doiron on Jun 2, 2015 3:48:40 PM

Over the years, a variety of protocols and methods have been developed for dissociation of different types of human cancer cells. One reason for the rise of different protocols was a 1989 breast cancer study that found a link between the types of cancer cellular subpopulations found in dissociated tumor samples and the method of dissociation used.

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A Method for Isolating Total Protein from FFPE Tissue

Posted by Luke Doiron on May 25, 2015 6:00:00 AM

Today, millions of Formalin-Fixed, Paraffin Embedded (FFPE) biospecimens are prepared and put in archival storage for use in future research projects. The abundance of these tissues make them a wonderful resource for biomedical research. Historically, FFPE tissue samples were used for histological analysis. However, today, specimens are often needed for molecular characterization of tumor tissues, and specifically for developing therapies based on the expression changes in proteins found in tumor tissues. Protein extraction from FFPE tissues has been a challenge because of the tight cross-link matrices formed during the fixation and embedding procedure.

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