4 Disease Research Tests that Require Diluting Whole Blood

Posted by Luke Doiron on Nov 18, 2014 6:00:00 AM


Human blood offers a rich mine of information for researchers studying disease origins and treatment possibilities. The human body contains about five liters of blood, representing roughly seven percent of overall body weight for an average-sized individual. Blood performs many critical functions, including supplying oxygen to tissues via erythrocytes; removal of wastes; and immune system disease-protection via leukocytes, most specifically lymphocytes and monocytes. Many disease research protocols require dilution of whole blood, often for subsequent purification of blood components. Let's look at a few procedures researchers have developed for investigating disease using dilute whole blood:

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What is the Typical Procedure for PBMC Isolation from Whole Blood

Posted by Luke Doiron on Nov 11, 2014 6:00:00 AM


The most common method for separating and PBMC isolation (peripheral blood mononuclear cells) - most specifically lymphocytes and monocytes - is a method known as Ficoll or Ficoll-Paque. This density gradient medium was developed by GE Healthcare based on the principle of differential migration of blood cells through the media during the centrifugation stage of the procedure.

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Materials and Equipment Needed for PBMC Isolation from Whole Blood

Posted by Luke Doiron on Nov 4, 2014 6:00:00 AM


Peripheral Blood Mononuclear Cells (PBMCs) are derived from whole blood and are commonly used biospecimens for immunology and cancer research. PBMCs are used because they are comprised primarily of lymphocytes and monocytes. Lymphocytes are made up of T and B cells, as well as NK cells. Each of these cells play a critical role in defending the body as a part of the immune system's defense mechanism. T cells are in charge of cellular immunity functions. B cells interact with antibodies and help confer what is known as antibody-mediated immunity. NK (natural killer) cells prowl the body looking for antigens with abnormal cell membranes, such as cancer cells. When an NK cell encounters these 'enemy cells', it releases a protein that attacks the abnormal cell's membrane. Lymphocytes make up over 25 percent of all white blood cells (WBCs) and are probably the most important cells in the human body's immune system. Monocytes are also key to a healthy immune system via their role in surrounding foreign particles that enter the body. This process is known as phagocytosis.

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The Importance of Bone Marrow Adult Stem Cells in Medicine

Posted by Luke Doiron on Oct 28, 2014 6:00:00 AM

We are born with a supply of bone marrow in the center of our large bones. This soft and spongy tissue is critical to the development of the immune system, as well as the source of new blood cells. At birth, all bone marrow is red, and consists primarily of hematopoietic tissue, key to the production of red and white blood cells, as well as platelets. Bone marrow stroma contains a number of other cells, including mesenchymal stem cells (MSCs), which have been found to be extremely valuable in disease research. Many cell progenitors are also found in bone marrow and are useful in helping researchers understand the root causes of blood and autoimmune diseases. Adult bone marrow stem cells are now also used in actual patient treatments for a variety of serious diseases.

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5 Benefits to Using Adult Stem Cells in Cancer Research

Posted by Luke Doiron on Oct 21, 2014 6:00:00 AM

While much of the popular media attention over the last 10 years has focused on embryonic stem cells, in fact, the adult stem cell has been shown to be a viable and valuable source in the long fight to better understand cancer's origins and treatment possibilities. Adult stem cells, in brief, are also known as progenitor cells or somatic stem cells. They are found in minute quantities in nearly every human body organ and tissue. Their key function is maintenance and repair of their specific tissues.

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6 Things to Know About Seropositive Rheumatoid Arthritis Research

Posted by Luke Doiron on Oct 14, 2014 6:00:00 AM

A chronic autoimmune disease, rheumatoid arthritis (RA), is theorized to be caused by some combination of genetics and environmental factors. What the precise molecular trigger is for RA is still under investigation so that better treatments can be developed and diagnosis made earlier, when the disease is more manageable. Early symptoms of RA include joint pain, swelling, and stiffness. As the disease progresses, it can lead to loss of mobility and joint deformity.

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How are Extracted Nucleic Acids Used for Drug Development?

Posted by Luke Doiron on Oct 9, 2014 6:00:00 AM

Before powerful modern microscopes enabled medical researchers to clearly see and define microscopic organisms such as bacteria and viruses, the earliest microbe hunters used a rudimentary magnifying glass to gain superficial knowledge about the tiny world of microbes. Today, we can plausibly compare advancements in molecular medicine to that journey from a simple magnifying lens to a modern microscope.

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5 Frequently Asked Questions About Adult Stem Cell Research

Posted by Luke Doiron on Oct 7, 2014 6:00:00 AM

Stem cells are often referred to in the sociopolitical realm with some level of controversy and beyond that, some level of confusion. Many researchers are unaware of the basic scientific definitions of various types of stem cells and their medical therapeutic potential applications. In order to have a better understanding of the adult stem cell, here are five frequently asked questions (FAQs) about adult stem cell research:

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