What Parameters Impact the Outcome of Viable Dissociated Cells

Posted by Luke Doiron on Jul 29, 2015 3:11:00 PM

When cell isolation is the goal, selecting the right protocol can greatly influence cell viability and yield, and therefore impact your research data. This is why it's important to understand what parameters effect the outcome of cell dissociation processes, so you select the correct materials and methods for your desired endpoint and downstream application.

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7 Steps for Successful Basic Primary Cell Isolation

Posted by Luke Doiron on Jul 22, 2015 2:07:36 PM

 

Primary cell isolation is required for subsequent generation of primary cell cultures, which are useful in-vitro tools for pre-clinical disease research. Common applications of primary cell isolates include studies of cellular communication and illumination of disease mechanisms, including cancer, autoimmune diseases, and diabetes.

Primary cells from a biospecimen are typically heterogeneous, so basic cell isolation is used to dissociate and separate the various cell types from tissue specimens. Many researchers believe that primary cells best represent the tumor biology and heterogeneity encountered in the actual clinical environment, and so are worthwhile and important in-vitro models.

When it comes to basic primary cell isolation, it's key to understand that there are a wide variety of parameters that can impact the outcome of any isolation procedure. These include the type of tissue, species origin, specimen age, genetic modification (i.e. knockouts), enzyme type and quality, and incubation time and temperature.

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Rheumatoid Arthritis: Causes and Symptoms

Posted by Madison Richey on Jul 16, 2015 4:30:00 PM

More than 1.5 million people in the United States have rheumatoid arthritis. About 70 percent of these are women. The immune system normally protects its health by attacking foreign substances like bacteria and viruses, but in rheumatoid arthritis patients, the body’s immune system mistakenly attacks the joints. This mistake creates inflammation that causes the tissue inside of the joints to thicken resulting in swelling and pain around the joints. If inflammation goes unchecked, it can damage the elastic tissue that covers the ends of bones in a joint and the bones themselves. Over time, there is loss of cartilage, and the joint spacing between bones can become smaller. Joints can become loose, unstable, and painful. Joint damage cannot be reversed.

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Crohn’s Disease: A Patient’s Perspective

Posted by Olivia Hendrick on Jul 15, 2015 8:00:00 AM

In 2008, my family and I found out that my 7-year-old sister, Jane Claire, had Crohn’s disease. She had been sick for a very long time and was exhibiting the typical symptoms of Crohn’s disease such as weight loss, reduced appetite, fatigue, abdominal cramps, and diarrhea. However, because Crohn’s disease is so hard to diagnose, it took about a year until the doctors could reach a definitive answer about the cause of her symptoms. Throughout the process she was told she was anemic, had lupus, and leukemia, and several other diseases. 

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8 Types of Enzymes for Tumor Cell Dissociation

Posted by Luke Doiron on Jul 7, 2015 12:00:00 PM

Cancer researchers understand that there is quite a bit of heterogeneity among the cells found in human tumor biospecimens. Such variations can result in cells that differ in growth properties, drug toxicity, immunological reactivity, and proliferative potential. For this reason, when tumor specimens are used for in-vitro studies, it's very important to properly isolate and dissociate all the cells from one another, so that researchers gain better understanding of tumor progression or regression properties, as well as predicting therapeutic responses to novel treatment modalities, including potential new drug regimens.

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Alzheimer's Disease: How do you know, and how do you cope?

Posted by Madison Richey on Jul 4, 2015 7:30:00 AM

We have been receiving feedback from readers stating that they would like to learn more about what patient's lives are like during the diagnosis and treatment phases of the diseases we work with.  Often, clues from treatment procedures will help indicate the availability of biospecimens. Over the next few weeks, look for a series of articles surrounding patient treatment and diagnosis as well as a few patient profiles.

Alzheimer's is a brain disease that causes a slow decline in memory, thinking and reasoning skills. There are ten warning signs from the Alzheimer’s Association that you may possibly be developing Alzheimer’s. Not all individuals will experience all of these signs, and some may experience them at varying degrees.

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How to Minimize Bleed-Through in Images of FFPE Sections

Posted by Luke Doiron on Jun 25, 2015 6:00:00 AM

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Is It Necessary to Thaw PBMCs in Their Expanding Media?

Posted by Luke Doiron on Jun 23, 2015 2:45:00 PM

Scientists conducting research in a wide variety of fields, including immunology, infectious diseases, stem cells, cancer, drug development, vaccine development, transplant immunology and cell-based assays often turn to PBMC specimens. Thousands of cryopreserved PBMC specimens are available in biobanks around the world, making them an important source for researchers. Fortunately, it's been found that cryopreserved  PBMCs are useful and valid specimens as long as they are properly handled.

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