Diabetes is a group of diseases marked by high levels of blood glucose resulting from defects in insulin production, insulin action, or both. Diabetes can lead to serious complications and premature death, but people with diabetes can take steps to control the disease and lower the risk of complications. Below are the most frequently asked questions about the disease:Read More
Peripheral blood mononuclear cells (PBMCs) contain primarily lymphocytes and monocytes and after their isolation from whole blood, can be used in a wide variety of critical in vitro studies. They find particular use in the fields of immunology, cell regeneration studies, cancer and drug discovery. Activation in various culture media is a necessary procedure for such studies as immunoglobulin production, lymphocyte/monocytes culturing, or isolation and expansion of infectious viruses such as HIV.Read More
The peripheral blood mononuclear cell (PBMC) is a most versatile biospecimen, containing a variety of cells that play a critical role in the healthy functioning of the body's immune system. PBMCs can be readily separated from whole blood specimens, and provide a reliable tool for studying a plethora of pathologic processes including infectious diseases, autoimmune disorders and cancer. In addition, studies show that PBMCs may be a useful source for regenerative therapies due to their ability to differentiate into multiple cell types.Read More
Isolation of human peripheral blood mononuclear cells (PBMC) is one of the most commonly performed tests for studying a wide range of human biological responses and diseases. For example, researchers who are looking into immune responses to vaccines or new immunotherapy modalities or are seeking a better understanding of the molecular basis of immune system function often source PBMC specimens. PBMCs find wide use in drug testing and target discovery, toxicology studies, cancer stem cell research, regenerative investigations and xenografting. Protocols often call for obtaining human PBMCs from both normal donors and from patients with diseases such as cancer or autoimmune/inflammatory conditions.Read More
Immunology is the study of how organisms defend the body from invading microorganisms.
Significant advances in the field include the identification of the two arms of immune responses known as 'innate' and 'adaptive.' Initially, innate immunity was considered to be a relatively nonspecific and simple part of the overall immune response, while adaptive immunity was believed to provide antigen-specific protection from microbial and viral infection. (1)
Researchers are interested in the inner workings of the immune system, particularly peripheral blood mononuclear cells (PBMCs), which include white blood cells, that are key responders to infection. They found a whole host of genes in these cells which varied in their transcription. Assessing gene expression profiles from readily available tissues, such as peripheral blood, would be useful and desirable in clinical practice. Importantly, the conversion of blood gene expression data into practical diagnostics could be a turning point in molecular medicine and personalized genomics. (2)Read More
The advancements in cancer drug discovery have really exploded in the last decade, but there have been specific crucial scientific breakthroughs that have changed the course of targeted cancer therapy and personalized medicine. The field of oncology is burgeoning and current research is addressing specific “tumors” instead of specific “cancers.” For instance, breast cancer is not just one type of cancer. There are 6 known types of breast cancers. The discovery of genetic mutations and biological influences of various tumors, sets the stage for a new field of clinical research in the area of targeted cancer therapy. This paradigm shift is due largely to the following discoveries:Read More